Structure of PDB 2wtx Chain A

Receptor sequence
>2wtxA (length=456) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
SRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQ
PLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRP
AWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIG
FFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNL
TRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAE
LKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSR
GDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRY
SDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIV
NPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDL
KQIVPR
3D structure
PDB2wtx Mechanistic Insight Into Enzymatic Glycosyl Transfer with Retention of Configuration Through Analysis of Glycomimetic Inhibitors.
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H154 D361
Catalytic site (residue number reindexed from 1) H154 D361
Enzyme Commision number 2.4.1.15: alpha,alpha-trehalose-phosphate synthase (UDP-forming).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 UDP A A20 G21 G22 R262 K267 F339 L344 L365 V366 E369 A20 G21 G22 R262 K267 F339 L344 L365 V366 E369
BS02 VDO A R9 G22 L23 Y76 W85 D130 H154 I225 R262 R300 D361 M363 N364 R9 G22 L23 Y76 W85 D130 H154 I225 R262 R300 D361 M363 N364 MOAD: Ki=1.3mM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity
GO:0016757 glycosyltransferase activity
GO:0016758 hexosyltransferase activity
Biological Process
GO:0005992 trehalose biosynthetic process
GO:0006950 response to stress
GO:0006970 response to osmotic stress
GO:0006974 DNA damage response
GO:0070415 trehalose metabolism in response to cold stress

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Molecular Function
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Biological Process
External links
PDB RCSB:2wtx, PDBe:2wtx, PDBj:2wtx
PDBsum2wtx
PubMed20077550
UniProtP31677|OTSA_ECOLI Trehalose-6-phosphate synthase (Gene Name=otsA)

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