Structure of PDB 2wtf Chain A

Receptor sequence

>2wtfA (length=509) Species: 4932 (Saccharomyces cerevisiae)

MSKFTWKELIQLGSPSKAYESSLACIAHIDMNAFFAQVEQMRCGLSKEDP
VVCVQWNSIIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFKKGED
FWQYHDGCGSWVQDPAKQISVEDHKVSLEPYRRESRKALKIFKSACDLVE
RASIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIGGNYD
INSHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCKGIRD
SIKDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLDCGKF
EITSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFLDAKV
KQSDYDRSTSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMMSNKN
LRGKSCNSIVDCISWLEVFCAELTSRIQDLEQEYNKIVIPRTVSISLKTK
SYEVYRKSGPVAYKGINFQSHELLKVGIKFVTDLDIKGKNKSYYPLTKLS
MTITNFDII

3D structure

• PDB: 2wtf Crystal Structure of a Cisplatin-(1,3-Gtg) Cross-Link within DNA Polymerase Eta.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S58 Y64 R67 R73 M74 K279 K393 M396 N398 R426	S58 Y64 R67 R73 M74 K279 K393 M396 N398 R426
BS02	dna	A	S153 G310 V311 R456	S153 G310 V311 R456
BS03	DTP	A	D30 N32 A33 F34 F35 I60 Y64 R73 D155 K279	D30 N32 A33 F34 F35 I60 Y64 R73 D155 K279
BS04	CA	A	D30 M31 D155	D30 M31 D155
BS05	CA	A	D30 D155 E156	D30 D155 E156
BS06	CA	A	D318 D321	D318 D321

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2wtf, PDBe:2wtf, PDBj:2wtf
• PDBsum: 2wtf
• PubMed: 20333640
• UniProt: Q04049|POLH_YEAST DNA polymerase eta (Gene Name=RAD30)