Structure of PDB 2wsd Chain A

Receptor sequence

>2wsdA (length=505) Species: 1423 (Bacillus subtilis)

TLEKFVDALPIPDTLKPVQQSKEKTYYEVTMEECTHQLHRDLPPTRLWGY
NGLFPGPTIEVKRNENVYVKWMNNLPSTHFLPIDHTIHHEEPEVKTVVHL
HGGVTPDDSDGYPEAWFSKDFEQTGPYFKREVYHYPNQQRGAILWYHDHA
MALTRLNVYAGLVGAYIIHDPKEKRLKLPSDEYDVPLLITDRTINEDGSL
FYPSAPENPSPSLPNPSIVPAFCGETILVNGKVWPYLEVEPRKYRFRVIN
ASNTRTYNLSLDNGGDFIQIGSDGGLLPRSVKLNSFSLAPAERYDIIIDF
TAYEGESIILANSAGCGGDVNPETDANIMQFRVTKPLAQKDESRKPKYLA
SYPSVQHERIQNIRTLKLAGTQDEYGRPVLLLNNKRWHDPVTETPKVGTT
EIWSIINPTRGTHPIHLHLVSFRVLDRRPFDIARYQESGELSYTGPAVPP
PPSEKGWKDTIQAHAGEVLRIAATFGPYSGRYVWHCHALEHEDYDMMRPM
DITDP

3D structure

• PDB: 2wsd Proximal Mutations at the Type 1 Copper Site of Cota Laccase: Spectroscopic, Redox, Kinetic and Structural Characterization of I494A and L386A Mutants.
• Chain: A
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 1.10.3.2: laccase.
  1.3.3.5: bilirubin oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU	A	H419 C492 H497 P511	H413 C486 H491 P505
BS02	CU	A	H107 H153 H493	H101 H147 H487
BS03	CU	A	H155 H424 H491	H149 H418 H485
BS04	CU	A	H105 H422 H424	H99 H416 H418
BS05	OXY	A	H105 H153 H155 H422 H491 H493	H99 H147 H149 H416 H485 H487

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0030435 sporulation resulting in formation of a cellular spore

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:2wsd, PDBe:2wsd, PDBj:2wsd
• PDBsum: 2wsd
• PubMed: 18307408
• UniProt: P07788|COTA_BACSU Laccase (Gene Name=cotA)