Structure of PDB 2wqd Chain A

Receptor sequence

>2wqdA (length=570) Species: 1280 (Staphylococcus aureus)

SKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEA
SKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENAN
AATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNP
SMIDESVVIVGNDLTPSDTAQLNKEFVQGFATNIGGRTSASAIMSRSLEI
PAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAYQDKRERYFAD
KKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEF
LYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPE
EMNPFLGYRAIRLSLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEF
REAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFF
SIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWT
GMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELA
NRAVDCATQEEVIELVNNYV

3D structure

• PDB: 2wqd Crystal Structure of Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System in the Dephosphorylated State.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D12 L106 A191 E433 S452 D457 C504 E524
• Catalytic site (residue number reindexed from 1): D11 L105 A190 E432 S451 D456 C503 E523
• Enzyme Commision number: 2.7.3.9: phosphoenolpyruvate--protein phosphotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	Y474 Q477	Y473 Q476

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0008965 phosphoenolpyruvate-protein phosphotransferase activity
• GO:0016301 kinase activity
• GO:0016772 transferase activity, transferring phosphorus-containing groups
• GO:0046872 metal ion binding

Biological Process

• GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system
• GO:0016310 phosphorylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2wqd, PDBe:2wqd, PDBj:2wqd
• PDBsum: 2wqd
• PubMed: 19801641
• UniProt: P51183|PT1_STAAU Phosphoenolpyruvate-protein phosphotransferase (Gene Name=ptsI)