Structure of PDB 2whx Chain A

Receptor sequence

>2whxA (length=582) Species: 11070 (dengue virus type 4)

GVYRIMQRGLFGKTQVGVGIHMEGVFHTMWHVTRGSVICHERLEPSWADV
RNDMISYGGGWRLGDKWDKEEDVQVLAIEPGKNPKHVQTKPGLFEIGAVT
LDFKPGTSGSPIINKKGKVIGLYGNVSAITQAERIGEPDYEVDEDIFRKK
RLTIMDLHPGAGKTKRILPSIVREALKRRLRTLILAPTRVVAAEMEEALR
GLPIRYQTPAVKSDHTGREIVDLMCHATFTTRLLSSTRVPNYNLIVMDEA
HFTDPCSVAARGYISTRVEMGEAAAIFMTATPPGSTDPFPQSNSPIEDIE
REIPERSWNTGFDWITDYQGKTVWFVPSIKAGNDIANCLRKSGKRVIQLS
RKTFDTEYPKTKLTDWDFVVTTDISEMGANFRAGRVIDPRRCLKPVILTD
GPERVILAGPIPVTPASAAQRRGRIGRNPAQEDDQYVFSGDPLKNDEDHA
HWTEAKMLLDNIYTPEGIIPTLFGPEREKTQAIDGEFRLRGEQRKTFVEL
MRRGDLPVWLSYKVASAGISYKDREWCFTGERNNQILEENMEVEIWTREG
EKKKLRPKWLDARVYADPMALKDFKEFASGRK

3D structure

• PDB: 2whx Flexibility between the Protease and Helicase Domains of the Dengue Virus Ns3 Protein Conferred by the Linker Region and its Functional Implications.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.91: flavivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	V22 Y23 R24 I25 M26 Q27 R28 F46 I58 C59 V95 Q96 I100 H108 Q110 I140 N141	V2 Y3 R4 I5 M6 Q7 R8 F26 I38 C39 V73 Q74 I78 H86 Q88 I113 N114
BS02	ADP	A	G196 G198 K199 T200 K201 R463	G160 G162 K163 T164 K165 R427
BS03	MN	A	T200 E285	T164 E249

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding

External links

• PDB: RCSB:2whx, PDBe:2whx, PDBj:2whx
• PDBsum: 2whx
• PubMed: 20375022
• UniProt: Q2YHF0|POLG_DEN4T Genome polyprotein