Structure of PDB 2w8s Chain A

Receptor sequence

>2w8sA (length=512) Species: 28094 (Trinickia caryophylli)

RKNVLLIVVDQWRADFIPHLMRAEGREPFLKTPNLDRLCREGLTFRNHVT
TCVPGGPARASLLTGLYLMNHRAVQNTVPLDQRHLNLGKALRAIGYDPAL
IGYTTTTPDPRTTSARDPRFTVLGDIMDGFRSVGAFEPNMEGYFGWVAQN
GFELPENREDIWLPEGEHSVPGATDKPSRIPKEFSDSTFFTERALTYLKG
RDGKPFFLHLGYYRPHPPFVASAPYHAMYKAEDMPAPIRAENPDAEAAQH
PLMKHYIDHIRRGSFFHGAEGSGATLDEGEIRQMRATYCGLITEIDDCLG
RVFAYLDETGQWDDTLIIFTSDHGEQLGDHHLLGKIGYNAESFRIPLVIK
DAGQNRHAGQIEEGFSESIDVMPTILEWLGGETPRACDGRSLLPFLAEGK
PSDWRTELHYEFDFRDVFYDQPQNSVQLSQDDCSLCVIEDENYKYVHFAA
LPPLFFDLKADPHEFSNLAGDPAYAALVRDYAQKALSWRLSHADRTLTHY
RSSPQGLTTRNH

3D structure

• PDB: 2w8s An Efficient, Multiply Promiscuous Hydrolase in the Alkaline Phosphatase Superfamily.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D12 Q13 G57 R61 Y105 T107 H218 D324 H325 K337
• Catalytic site (residue number reindexed from 1): D10 Q11 G55 R59 Y103 T105 H216 D322 H323 K335
• Enzyme Commision number: 3.1.-.-
  3.1.3.-
  3.1.4.-
  3.1.6.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	D12 X57 D324 H325	D10 X55 D322 H323
BS02	FE	A	D12 X57 D324 H325	D10 X55 D322 H323

Gene Ontology

Molecular Function

• GO:0008484 sulfuric ester hydrolase activity
• GO:0016787 hydrolase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2w8s, PDBe:2w8s, PDBj:2w8s
• PDBsum: 2w8s
• PubMed: 20133613
• UniProt: Q45087|BCPMH_TRICW Multifunctional alkaline phosphatase superfamily protein PehA (Gene Name=pehA)