Structure of PDB 2w7p Chain A

Receptor sequence

>2w7pA (length=431) Species: 9606 (Homo sapiens)

KEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKA
QLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGS
MSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVK
EILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKNSVVFGTS
AQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNR
QAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSE
TSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELC
SELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAK
ELLKTEIDADFPHPLRLRLMGVRISSFPNEE

3D structure

• PDB: 2w7p Structural and Functional Elucidation of the Mechanism Promoting Error-Prone Synthesis by Human DNA Polymerase Kappa Opposite the 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine Adduct.
• Chain: A
• Resolution: 3.71 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S196 D198 E199 K321 G356 I357 G358 K359 V360 T361 K468 T469 R470 T473	S164 D166 E167 K232 G267 I268 G269 K270 V271 T272 K379 T380 R381 T384
BS02	dna	A	T44 F49 M135 P153 F155 I156 S388 S391 R413 K414 S415 M416 S417 V418 E419 R420 T421 R507	T12 F17 M103 P121 F123 I124 S299 S302 R324 K325 S326 M327 S328 V329 E330 R331 T332 R418
BS03	DTP	A	D107 A110 S137 T138 Y141 R144 A150 D198 K328	D75 A78 S105 T106 Y109 R112 A118 D166 K239
BS04	CA	A	D107 D325	D75 D236
BS05	CA	A	M108 D198	M76 D166

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2w7p, PDBe:2w7p, PDBj:2w7p
• PDBsum: 2w7p
• PubMed: 19542228
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)