Structure of PDB 2vzm Chain A

Receptor sequence

>2vzmA (length=395) Species: 54571 (Streptomyces venezuelae)

PPVLDLGALGQDFAADPYPTYARLRAEGPAHRVRTPEGNEVWLVVGYDRA
RAVLADPRFSKDWRNSTTPLTEAEAALNHNMLESDPPRHTRLRKLVAREF
TMRRVELLRPRVQEIVDGLVDAMLAAPDGRADLMESLAWPLPITVISELL
GVPEPDRAAFRVWTDAFVFPDDPAQAQTAMAEMSGYLSRLIDSKRGQDGE
DLLSALVRTSDEDGSRLTSEELLGMAHILLVAGHETTVNLIANGMYALLS
HPDQLAALRADMTLLDGAVEEMLRYEGPVESATYRFPVEPVDLDGTVIPA
GDTVLVVLADAHRTPERFPDPHRFDIRRDTAGHLAFGHGIHFCIGAPLAR
LEARIAVRALLERCPDLALDVSPGELVWYPNPMIRGLKALPIRWR

3D structure

• PDB: 2vzm Analysis of Transient and Catalytic Desosamine Binding Pockets in Cytochrome P450 Pikc from Streptomyces Venezuelae.
• Chain: A
• Resolution: 1.85 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D23 P47 F180 A243 E246 T247 T248 V290 C354 I355 G356 E363 I395
• Catalytic site (residue number reindexed from 1): D12 P36 F169 A232 E235 T236 T237 V279 C343 I344 G345 E352 I384
• Enzyme Commision number: 1.14.15.33: pikromycin synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	M92 L93 H100 R104 A243 G244 T247 T248 L251 T294 R296 A346 F347 H352 C354 I355 G356 A360	M81 L82 H89 R93 A232 G233 T236 T237 L240 T283 R285 A335 F336 H341 C343 I344 G345 A349
BS02	NRB	A	F178 M191 I239 T247 I395	F167 M180 I228 T236 I384	MOAD: Kd=171.9uM

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0017000 antibiotic biosynthetic process
• GO:0033068 macrolide biosynthetic process

External links

• PDB: RCSB:2vzm, PDBe:2vzm, PDBj:2vzm
• PDBsum: 2vzm
• PubMed: 19124459
• UniProt: O87605|PIKC_STRVZ Cytochrome P450 monooxygenase PikC (Gene Name=pikC)