Structure of PDB 2vs8 Chain A

Receptor sequence

>2vs8A (length=183) [Search protein sequence]

ENVSGISAYLLGLIIGDGGLYKLKYKGNRSEYRVVITQKSENLIKQHIAP
LMQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMR
EQIAFIKGLYVAEGDKTLKRLRIWNKNKALLEIVSRWLNNLGVRNTIHLD
DHRHGVYVLNISLRDRIKFVHTILSSHLNPLPP

3D structure

PDB

2vs8 Crystal Structure of I-Dmoi in Complex with its Target DNA Provides New Insights Into Meganuclease Engineering.

Chain

Resolution

2.1 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	D21 T41 Q42 K43 T76 R77 R124 T150 H152 D154 R157 S166 L167	D17 T37 Q38 K39 T72 R73 R120 T146 H148 D150 R153 S162 L163
BS02	dna	A	G20 D21 G22 Y25 Y29 K30 R33 R37 R77	G16 D17 G18 Y21 Y25 K26 R29 R33 R73
BS03	dna	A	N32 R33 S34 E35 Y36 R37 S67 K68 Q70 R81 S83 S84 K85 E117 W128 N129 K130 D155 H158 V160	N28 R29 S30 E31 Y32 R33 S63 K64 Q66 R77 S79 S80 K81 E113 W124 N125 K126 D151 H154 V156
BS04	dna	A	A116 E117 K120 R126 W128	A112 E113 K116 R122 W124
BS05	dna	A	K86 Y89 N93	K82 Y85 N89
BS06	MN	A	D21 A116	D17 A112
BS07	MN	A	G20 E117	G16 E113

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

	Biological Process
GO:0006314	intron homing
GO:0016539	intein-mediated protein splicing

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Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:2vs8, PDBe:2vs8, PDBj:2vs8
PDBsum	2vs8
PubMed	18974222
UniProt	P21505\|DMO1_DESMO Homing endonuclease I-DmoI

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