Structure of PDB 2rj7 Chain A

Receptor sequence

>2rj7A (length=292) Species: 9606 (Homo sapiens)

FMVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNIDILNEQFRL
QNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVPR
VTLGTGRQLSVLEVRAYKRWQDVSMRRMEMISFCERRFLSEVDYLVCVDV
DMEFRDHVGVEILTPLFGTLHPGFYGSSREAFTYERRPQSQAYIPKDEGD
FYYMGAFFGGSVQEVQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLR
HKPTKVLSPEYLWDQQLLGWPAVLRKLRFTAVPKNHQAVRNP

3D structure

• PDB: 2rj7 ABO(H) blood group A and B glycosyltransferases recognize substrate via specific conformational changes.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H233 M266 W300 E303 A343 R352
• Catalytic site (residue number reindexed from 1): H171 M204 W238 E241 A281 R290
• Enzyme Commision number: 2.4.1.37: fucosylgalactoside 3-alpha-galactosyltransferase.
  2.4.1.40: glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GDU	A	F121 A122 I123 Y126 V184 S185 R188 D211 V212 D213 G267 A268 H301 D302 K346 R352	F60 A61 I62 Y65 V123 S124 R127 D149 V150 D151 G205 A206 H239 D240 K284 R290
BS02	MN	A	D211 D213	D149 D151
BS03	DA8	A	H233 F236 T245 W300 E303 D326 H348	H171 F174 T183 W238 E241 D264 H286

Gene Ontology

Molecular Function

• GO:0016758 hexosyltransferase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:2rj7, PDBe:2rj7, PDBj:2rj7
• PDBsum: 2rj7
• PubMed: 18192272
• UniProt: P16442|BGAT_HUMAN Histo-blood group ABO system transferase (Gene Name=ABO)