Structure of PDB 2rd2 Chain A

Receptor sequence

>2rd2A (length=529) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

TNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDY
KGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLH
AYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALF
EKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCI
YPMYDFTHCISDALEGITHSLRTLEFQDNRRLYDWVLDNITIPVHPRQYE
FSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIR
EFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQ
GEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGK
EVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLGVIHWVSAAHALPVE
IRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQF
EREGYFCLDSRHSTAEKPVFNRTVGLRDT

3D structure

PDB

2rd2 A rationally engineered misacylating aminoacyl-tRNA synthetase.

Chain

Resolution

2.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

6.1.1.18: glutamine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q13 E34 T68 N69 P126 R130 R133 T135 L136 G168 C171 P181 F182 I183 V189 R192 K194 M210 Y211 F233 D235 I313 T316 K317 Q318 N320 T321 E323 S326 S329 R341 P369 N370 Q399 K401 R402 R410 R412 N413 L442 Q517 E519 R520 R545	Q6 E27 T61 N62 P119 R123 R126 T128 L129 G161 C164 P174 F175 I176 V182 R185 K187 M203 Y204 F226 D228 I306 T309 K310 Q311 N313 T314 E316 S319 S322 R334 P362 N363 Q392 K394 R395 R403 R405 N406 L435 Q499 E501 R502 R527
BS02	QSI	A	P32 P33 G42 H43 Y211 H215 R229 T230 F233 R260 L261 K270	P25 P26 G35 H36 Y204 H208 R222 T223 F226 R253 L254 K263

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004819	glutamine-tRNA ligase activity
GO:0005524	ATP binding

	Biological Process
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006424	glutamyl-tRNA aminoacylation
GO:0006425	glutaminyl-tRNA aminoacylation
GO:0043039	tRNA aminoacylation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2rd2, PDBe:2rd2, PDBj:2rd2
PDBsum	2rd2
PubMed	18477696
UniProt	P00962\|SYQ_ECOLI Glutamine--tRNA ligase (Gene Name=glnS)

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