Structure of PDB 2qwb Chain A

Receptor sequence

>2qwbA (length=388) Species: 11320 (Influenza A virus)

RDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRF
YALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSS
TSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESEC
VCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSC
YGERAEITCTCKDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPND
PTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPN
ALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGR
PKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL

3D structure

• PDB: 2qwb Drug design against a shifting target: a structural basis for resistance to inhibitors in a variant of influenza virus neuraminidase.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D151 E277 K292 R371 Y406
• Catalytic site (residue number reindexed from 1): D70 E197 K212 R290 Y324
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SIA	A	R118 E119 D151 A246 E276 K292 R371 Y406	R37 E38 D70 A166 E196 K212 R290 Y324	MOAD: Ki=1820uM PDBbind-CN: -logKd/Ki=2.74,Ki=1820uM
BS02	SIA	A	S367 S370 S372 N400 D402 W403 K432	S286 S289 S291 N318 D320 W321 K352	MOAD: Ki=1820uM PDBbind-CN: -logKd/Ki=2.74,Ki=1820uM
BS03	CA	A	D293 N294 G297 D324 N347	D213 N214 G217 D244 N266

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0046761 viral budding from plasma membrane

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:2qwb, PDBe:2qwb, PDBj:2qwb
• PDBsum: 2qwb
• PubMed: 9655825
• UniProt: P03472|NRAM_I75A5 Neuraminidase (Gene Name=NA)