Structure of PDB 2q8m Chain A

Receptor sequence
>2q8mA (length=310) Species: 621 (Shigella boydii) [Search protein sequence]
MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAGLFANEK
LKAALKARDIVAGIASEEEDEIVVFEGCEHAKYVVLMDPLDGSSNIDVNV
SVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVVYGSSTMLVYTTG
CGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVKKYIK
FCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRL
LYECNPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDV
ERFIREFPDA
3D structure
PDB2q8m Structure of Inhibited Fructose-1,6-bisphosphatase from Escherichia coli: DISTINCT ALLOSTERIC INHIBITION SITES FOR AMP AND GLUCOSE 6-PHOSPHATE AND THE CHARACTERIZATION OF A GLUCONEOGENIC SWITCH.
ChainA
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E89 E90 D110 L112 D113 E275
Catalytic site (residue number reindexed from 1) E67 E68 D88 L90 D91 E253
Enzyme Commision number 3.1.3.11: fructose-bisphosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 BG6 A Y210 I221 K222 Q225 Y188 I199 K200 Q203 MOAD: Ki~700uM
BS02 FBP A D110 D113 G114 N206 Y239 L243 Y257 Y259 K269 E275 D88 D91 G92 N184 Y217 L221 Y235 Y237 K247 E253 MOAD: ic50=6uM
BS03 MG A E89 D110 L112 E67 D88 L90
BS04 MG A D110 D113 E275 D88 D91 E253
BS05 AMP A I8 Q12 F15 A18 T19 G20 E21 L22 K104 Y105 I8 Q12 F15 A18 T19 G20 E21 L22 K82 Y83 MOAD: Ki=94uM
PDBbind-CN: -logKd/Ki=4.03,Ki=94uM
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0042132 fructose 1,6-bisphosphate 1-phosphatase activity
GO:0042578 phosphoric ester hydrolase activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0005986 sucrose biosynthetic process
GO:0006000 fructose metabolic process
GO:0006002 fructose 6-phosphate metabolic process
GO:0006094 gluconeogenesis
GO:0030388 fructose 1,6-bisphosphate metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2q8m, PDBe:2q8m, PDBj:2q8m
PDBsum2q8m
PubMed17567577
UniProtP0A993|F16PA_ECOLI Fructose-1,6-bisphosphatase class 1 (Gene Name=fbp)

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