Structure of PDB 2q66 Chain A

Receptor sequence

>2q66A (length=519) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

KVFGITGPVSTVGATAAENKLNDSLIQELKKEGSFETEQETANRVQVLKI
LQELAQRFVYEVSKKKNMSDGMARDAGGKIFTYGSYRLGVHGPGSDIDTL
VVVPKHVTREDFFTVFDSLLRERKELDEIAPVPDAFVPIIKIKFSGISIA
LICARLDQPQVPLSLTLSDKNLLRNLDEKDLRALNGTRVTDEILELVPKP
NVFRIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACSAV
ILNRFFIILSEWNWPQPVILKPIEDGPLQVRVWNPKIYAQDRSHRMPVIT
PAYPSMCATHNITESTKKVILQEFVRGVQITNDIFSNKKSWANLFEKNDF
FFRYKFYLEITAYTRGSDEQHLKWSGLVESKVRLLVMKLEVLAGIKIAHP
FTKPFESSYCCPTEDDYEMIQDKYGSHKTETALNALKLVTDENEEESIKD
APKAYLSTMYIGLDFNIKVDIHIPCTEFVNLCRSFNEDYGDHKVFNLALR
FVKGYDLPDEVFDENEKRP

3D structure

PDB

2q66 Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis.

Chain

Resolution

1.8 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	S89 D100 D102 A154 Y224
Catalytic site (residue number reindexed from 1)	S85 D96 D98 A150 Y220
Enzyme Commision number	2.7.7.19: polynucleotide adenylyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	A	Y87 D102 V136 A139 F140 V141 I143 K145 N226 I227 Q294 H314 N315 G380 L381 S384 R387 K392 L491	Y83 D98 V132 A135 F136 V137 I139 K141 N222 I223 Q290 H310 N311 G376 L377 S380 R383 K388 L481
BS02	MG	A	D100 D102	D96 D98
BS03	ATP	A	Y87 G88 S89 D100 D102 K215 Y224 G233 V234 M310 A312	Y83 G84 S85 D96 D98 K211 Y220 G229 V230 M306 A308

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016740	transferase activity
GO:0016779	nucleotidyltransferase activity
GO:0046872	metal ion binding
GO:1990817	poly(A) RNA polymerase activity

	Biological Process
GO:0006397	mRNA processing
GO:0030846	termination of RNA polymerase II transcription, poly(A)-coupled
GO:0031123	RNA 3'-end processing
GO:0031124	mRNA 3'-end processing
GO:0031126	sno(s)RNA 3'-end processing

	Cellular Component
GO:0005634	nucleus
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2q66, PDBe:2q66, PDBj:2q66
PDBsum	2q66
PubMed	17850751
UniProt	P29468\|PAP_YEAST Poly(A) polymerase (Gene Name=PAP1)

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