Structure of PDB 2puf Chain A

Receptor sequence
>2pufA (length=338) Species: 562 (Escherichia coli) [Search protein sequence]
TIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVAR
SLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKA
DFTDAVIDNAFEGGYMAGRYLIERGHREIGVIPGPLEQNTGAGRLAGFMK
AMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAM
GALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAF
NMLLDRIVNKREEPQSIEVHPRLIERRSVADGPFRDYR
3D structure
PDB2puf Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190.
ChainA
Resolution3.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A S14 T16 T17 R26 A29 T32 L54 K55 S12 T14 T15 R24 A27 T30 L52 K53 PDBbind-CN: Kd=2.0uM
BS02 GUN A Y73 F74 Q190 T192 R196 F221 D275 Y71 F72 Q188 T190 R194 F219 D273
Gene Ontology
Molecular Function
GO:0000976 transcription cis-regulatory region binding
GO:0001217 DNA-binding transcription repressor activity
GO:0002057 guanine binding
GO:0003677 DNA binding
GO:0003700 DNA-binding transcription factor activity
GO:0005515 protein binding
GO:0042803 protein homodimerization activity
Biological Process
GO:0006164 purine nucleotide biosynthetic process
GO:0006355 regulation of DNA-templated transcription
GO:0045892 negative regulation of DNA-templated transcription
GO:1900372 negative regulation of purine nucleotide biosynthetic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:2puf, PDBe:2puf, PDBj:2puf
PDBsum2puf
PubMed9454587
UniProtP0ACP7|PURR_ECOLI HTH-type transcriptional repressor PurR (Gene Name=purR)

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