Structure of PDB 2pi5 Chain A

Receptor sequence
>2pi5A (length=862) Species: 10760 (Escherichia phage T7) [Search protein sequence]
IAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMF
PLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKT
TLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLN
KRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLI
ESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPC
VVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAIN
IAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNP
EALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMD
WRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVD
KVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGV
QHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIY
GIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWL
AYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPN
QAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRK
RCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDA
HKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADA
ANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
LRDILESDFAFA
3D structure
PDB2pi5 Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase.
ChainA
Resolution2.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A R96 G97 R99 Q135 S139 K206 V237 D240 E242 W422 Q744 R746 F755 R756 L757 Q758 T760 N762 R75 G76 R78 Q114 S118 K185 V216 D219 E221 W401 Q723 R725 F734 R735 L736 Q737 T739 N741
BS02 dna A R96 K98 P100 H211 R215 L747 N748 R75 K77 P79 H190 R194 L726 N727
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0019083 viral transcription
GO:0039695 DNA-templated viral transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2pi5, PDBe:2pi5, PDBj:2pi5
PDBsum2pi5
PubMed17512007
UniProtP00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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