Structure of PDB 2owc Chain A

Receptor sequence

>2owcA (length=498) Species: 274 (Thermus thermophilus)

MELPRAFGLLLHPTSLPGPYGVGVLGQEARDFLRFLKEAGGRYWQVLPLG
PTGYGDSPYQSFSAFAGNPYLIDLRPLAERGYVRLEDPGFPQGRVDYGLL
YAWKWPALKEAFRGFKEKASPEEREAFAAFREREAWWLEDYALFMALKGA
HGGLPWNRWPLPLRKREEKALREAKSALAEEVAFHAFTQWLFFRQWGALK
AEAEALGIRIIGDMPIFVAEDSAEVWAHPEWFHLDEEGRPTVVAGVPPDY
FSETGQRWGNPLYRWDVLEREGFSFWIRRLEKALELFHLVRIDHFRGFEA
YWEIPASCPTAVEGRWVKAPGEKLFQKIQEVFGEVPVLAEDLGVITPEVE
ALRDRFGLPGMKVLQFAFDMENPFLPHNYPAHGRVVVYTGTHDNDTTLGW
YRTATPHEKAFMARYLADWGITFREEEEVPWALMHLGMKSVARLAVYPVQ
DVLALGSEARMNYPGRPSGNWAWRLLPGELSPEHGARLRAMAEATERL

3D structure

• PDB: 2owc Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase.
• Chain: A
• Resolution: 2.05 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D293 E340 N396
• Catalytic site (residue number reindexed from 1): D293 E340 N394
• Enzyme Commision number: 2.4.1.25: 4-alpha-glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	Y59 D293 H394 D395	Y59 D293 H392 D393
BS02	AC1	A	S57 Q60 R257 W258 N464 P466	S57 Q60 R257 W258 N462 P464

Gene Ontology

Molecular Function

• GO:0004134 4-alpha-glucanotransferase activity
• GO:0016757 glycosyltransferase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2owc, PDBe:2owc, PDBj:2owc
• PDBsum: 2owc
• PubMed: 17420245
• UniProt: O87172|MALQ_THETH 4-alpha-glucanotransferase (Gene Name=malQ)