Structure of PDB 2oa1 Chain A

Receptor sequence

>2oa1A (length=527) Species: 68170 (Lentzea aerocolonigenes)

SGKIDKILIVGGGTAGWMAASYLGKALQGTADITLLQAPDIPTLGVGEAT
IPNLQTAFFDFLGIPEDEWMRECNASYKVAIKFINWRTAGEGTSEARELD
GGPDHFYHSFGLLKYHEQIPLSHYWFDRSYRGKTVEPFDYACYKEPVILD
ANRSPRRLDGSKVTNYAWHFDAHLVADFLRRFATEKLGVRHVEDRVEHVQ
RDANGNIESVRTATGRVFDADLFVDCSGFRGLLINKAMEEPFLDMSDHLL
NDSAVATQVPHDDDANGVEPFTSAIAMKSGWTWKIPMLGRFGTGYVYSSR
FATEDEAVREFCEMWHLDPETQPLNRIRFRVGRNRRAWVGNCVSIGTSSC
FVEPLESTGIYFVYAALYQLVKHFPDKSLNPVLTARFNREIETMFDDTRD
FIQAHFYFSPRTDTPFWRANKELRLADGMQEKIDMYRAGMAINAPASDDA
QLYYGNFEEEFRNFWNNSNYYCVLAGLGLVPDAPSPRLAHMPQATESVDE
VFGAVKDRQRNLLETLPSLHEFLRQQH

3D structure

• PDB: 2oa1 The structure of flavin-dependent tryptophan 7-halogenase RebH.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Enzyme Commision number: 1.14.19.9: tryptophan 7-halogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TRP	A	I52 H109 S110 F111 E357 Y455 E461 F465 W466	I51 H108 S109 F110 E356 Y454 E460 F464 W465
BS02	FAD	A	G12 G13 T15 A16 A39 E49 A50 R196 V197 C227 S228 W284 F330 T348 F352 P355 G360 I361	G11 G12 T14 A15 A38 E48 A49 R195 V196 C226 S227 W283 F329 T347 F351 P354 G359 I360

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004497 monooxygenase activity
• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:2oa1, PDBe:2oa1, PDBj:2oa1
• PDBsum: 2oa1
• PubMed: 17876823
• UniProt: Q8KHZ8|TRP7H_LENAE Tryptophan 7-halogenase RebH (Gene Name=rebH)