Structure of PDB 2o59 Chain A

Receptor sequence
>2o59A (length=619) Species: 562 (Escherichia coli) [Search protein sequence]
MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQ
PDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVH
AGDPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLR
SNSEFVPLCVSALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQT
PVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIWQPSCEPYQDE
EGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIEAA
KRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNAIS
VHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEAKV
YNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLLGS
KNDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFV
QDKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKAL
FHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAK
RTPVRQFRGIGAIAHHHHH
3D structure
PDB2o59 Structural Studies of E. coli Topoisomerase III-DNA Complexes Reveal a Novel Type IA Topoisomerase-DNA Conformational Intermediate.
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E7 D105 E107
Catalytic site (residue number reindexed from 1) E7 D105 E107
Enzyme Commision number 5.6.2.1: DNA topoisomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A Q50 P51 W61 E107 R165 D169 W170 G173 I174 T177 R178 R185 S194 G196 R197 V198 Q199 R330 T524 T527 R538 Q50 P51 W61 E107 R165 D169 W170 G173 I174 T177 R178 R185 S194 G196 R197 V198 Q199 R328 T517 T520 R531
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003916 DNA topoisomerase activity
GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
GO:0005515 protein binding
GO:0046872 metal ion binding
GO:0098847 sequence-specific single stranded DNA binding
Biological Process
GO:0006261 DNA-templated DNA replication
GO:0006265 DNA topological change
GO:0006281 DNA repair
GO:0006310 DNA recombination
GO:0051304 chromosome separation
Cellular Component
GO:0043597 cytoplasmic replication fork

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2o59, PDBe:2o59, PDBj:2o59
PDBsum2o59
PubMed17331537
UniProtP14294|TOP3_ECOLI DNA topoisomerase 3 (Gene Name=topB)

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