Structure of PDB 2nzt Chain A

Receptor sequence

>2nztA (length=871) Species: 9606 (Homo sapiens) [Search protein sequence]

DQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPT
FVRSTPDGTEHGEFLALDLGGTNFRVLWVKVVEMENQIYAIPEDIMRGSG
TQLFDHIAECLANFMDKLQIKDKKLPLGFTFSFPCHQTKLDESFLVSWTK
GFKSSGVEGRDVVALIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDHN
CEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGSLNDI
RTEFDQEIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFGGK
LSPELLNTGRFETKDISDIEGEKDGIRKAREVLMRLGLDPTQEDCVATHR
ICQIVSTRSASLCAATLAAVLQRIKENKGEERLRSTIGVDGSVYKKHPHF
AKRLHKTVRRLVPGCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKT
LEHLQLSHDQLLEVKRRMKVEMERGLSKETHASAPVKMLPTYVCGDFLAL
DLGGTNFRVLLVRVRGVEMHNKIYAIPQEVMHGTGDELFDHIVQCIADFL
EYMGMSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEGEDVVTLLKE
AIHRRDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGSNACYMEEMRN
VELVEGEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQRFE
KMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIES
DCLALLQVRAILQHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVV
DRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQ
SEDGSGKGAALITAVACRIRE

3D structure

PDB

2nzt The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation.

Chain

Resolution

2.45 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R539 S603 K618 D657
Catalytic site (residue number reindexed from 1)	R508 S564 K579 D615
Enzyme Commision number	2.7.1.1: hexokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLC	A	S155 F156 T172 K173 N208 D209 N235 E260 E294	S132 F133 T149 K150 N185 D186 N212 E237 E271
BS02	BG6	A	D84 G87 T88 D209 G231 T232 D413 S415 S449	D68 G71 T72 D186 G208 T209 D390 S392 S426
BS03	GLC	A	S603 F604 T620 K621 N656 D657 I677 N683 E708 E742	S564 F565 T581 K582 N614 D615 I635 N641 E666 E700
BS04	BG6	A	D532 G535 T536 D657 T680 D861 G862 T863 S897	D501 G504 T505 D615 T638 D819 G820 T821 S855

Gene Ontology

	Molecular Function
GO:0004340	glucokinase activity
GO:0004396	hexokinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0005536	D-glucose binding
GO:0008865	fructokinase activity
GO:0016301	kinase activity
GO:0016773	phosphotransferase activity, alcohol group as acceptor

	Biological Process
GO:0001666	response to hypoxia
GO:0001678	intracellular glucose homeostasis
GO:0002931	response to ischemia
GO:0005975	carbohydrate metabolic process
GO:0006002	fructose 6-phosphate metabolic process
GO:0006006	glucose metabolic process
GO:0006096	glycolytic process
GO:0007595	lactation
GO:0008637	apoptotic mitochondrial changes
GO:0016310	phosphorylation
GO:0019318	hexose metabolic process
GO:0035795	negative regulation of mitochondrial membrane permeability
GO:0045766	positive regulation of angiogenesis
GO:0046324	regulation of D-glucose import
GO:0046835	carbohydrate phosphorylation
GO:0051156	glucose 6-phosphate metabolic process
GO:0061621	canonical glycolysis
GO:0072655	establishment of protein localization to mitochondrion
GO:0072656	maintenance of protein location in mitochondrion
GO:1901135	carbohydrate derivative metabolic process
GO:1990830	cellular response to leukemia inhibitory factor
GO:2000378	negative regulation of reactive oxygen species metabolic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005741	mitochondrial outer membrane
GO:0005813	centrosome
GO:0005829	cytosol
GO:0016020	membrane
GO:0016529	sarcoplasmic reticulum
GO:0043231	intracellular membrane-bounded organelle

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2nzt, PDBe:2nzt, PDBj:2nzt
PDBsum	2nzt
PubMed	29298880
UniProt	P52789\|HXK2_HUMAN Hexokinase-2 (Gene Name=HK2)

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