Structure of PDB 2jkc Chain A

Receptor sequence

>2jkcA (length=516) Species: 294 (Pseudomonas fluorescens)

NKPIKNIVIVGGGTAGWMAASYLVRALQQQANITLIESAAIPRIGVGEAT
IPSLQKVFFDFLGIPEREWMPQVNGAFKAAIKFVNWRKSPDPSRDDHFYH
LFGNVPNCDGVPLTHYWLRKREQGFQQPMEYACYPQPGALDGKLAPCLSD
GTRQMSHAWHFDAHLVADFLKRWAVERGVNRVVDEVVDVRLNNRGYISNL
LTKEGRTLEADLFIDCSGMRGLLINQALKEPFIDMSDYLLCDSAVASAVP
NDDARDGVEPYTSSIAMNSGWTWKIPMLGRFGSGYVFSSHFTSRDQATAD
FLKLWGLSDNQPLNQIKFRVGRNKRAWVNNCVSIGLSSCFLEPLDSTGIY
FIYAALYQLVKHFPDTSFDPRLSDAFNAEIVHMFDDCRDFVQAHYFTTSR
DDTPFWLANRHDLRLSDAIKEKVQRYKAGLPLTTTSFDDSTYYETFDYEF
KNFWLNGNYYCIFAGLGMLPDRSLPLLQHRPESIEKAEAMFASIRREAER
LRTSLPTNYDYLRSLR

3D structure

• PDB: 2jkc New insights into the mechanism of enzymatic chlorination of tryptophan.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 1.14.19.9: tryptophan 7-halogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TRP	A	I52 H101 F103 D346 Y444 E450 F454 W455	I51 H100 F102 D345 Y443 E449 F453 W454
BS02	FAD	A	G12 G13 G14 A16 S39 I42 R44 I45 V47 G48 A50 E186 V187 C217 S218 G336 L337 F341 P344 I350	G11 G12 G13 A15 S38 I41 R43 I44 V46 G47 A49 E185 V186 C216 S217 G335 L336 F340 P343 I349

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004497 monooxygenase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0017000 antibiotic biosynthetic process

External links

• PDB: RCSB:2jkc, PDBe:2jkc, PDBj:2jkc
• PDBsum: 2jkc
• PubMed: 18979475
• UniProt: P95480|TRP7H_PSEFL Tryptophan 7-halogenase PrnA (Gene Name=prnA)