Structure of PDB 2iw4 Chain A

Receptor sequence
>2iw4A (length=307) Species: 1423 (Bacillus subtilis) [Search protein sequence]
EKILIFGHQNPDTDTICSAIAYADLKNKLGFNAEPVRLGQVNGETQYALD
YFKQESPRLVETAANEVNGVILVDHNERQQSIKDIEEVQVLEVIDHQRIA
NFETAEPLYYRAEPVGCTATILNKMYKENNVKIEKEIAGLMLSAIISDSL
LFKSPTCTDQDVAAAKELAEIAGVDAEEYGLNMLKAGADLSKKTVEELIS
LDAKEFTLGSKKVEIAQVNTVDIEDVKKRQAELEAVISKVVAEKNLDLFL
LVITDILENDSLALAIGNEAAKVEKAFNVTLENNTALLKGVVSRKKQVVP
VLTDAMA
3D structure
PDB2iw4 A Trimetal Site and Substrate Distortion in a Family II Inorganic Pyrophosphatase.
ChainA
Resolution2.15 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.6.1.1: inorganic diphosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE A H9 D13 D75 H8 D12 D74
BS02 FE A D15 D75 H97 D149 D14 D74 H96 D148
BS03 MG A D13 D149 D12 D148
BS04 MN A H9 D13 D75 H8 D12 D74
BS05 MN A D15 D75 H97 D149 D14 D74 H96 D148
BS06 2PN A D13 H97 Q98 D149 K205 R295 K296 D12 H96 Q97 D148 K204 R294 K295 MOAD: Kd=12uM
Gene Ontology
Molecular Function
GO:0004427 inorganic diphosphate phosphatase activity
GO:0016462 pyrophosphatase activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Cellular Component
External links
PDB RCSB:2iw4, PDBe:2iw4, PDBj:2iw4
PDBsum2iw4
PubMed17095506
UniProtP37487|PPAC_BACSU Manganese-dependent inorganic pyrophosphatase (Gene Name=ppaC)

[Back to BioLiP]