Structure of PDB 2ivp Chain A

Receptor sequence

>2ivpA (length=325) Species: 29292 (Pyrococcus abyssi)

MLALGIEGTAHTLGIGIVSEDKVLANVFDTLTTEKGGIHPKEAAEHHARL
MKPLLRKALSEAGVSLDDIDVIAFSQGPGLGPALRVVATAARALAVKYRK
PIVGVNHCIAHVEITKMFGVKDPVGLYVSGGNTQVLALEGGRYRVFGETL
DIGIGNAIDVFARELGLGFPGGPKVEKLAEKGEKYIELPYAVKGMDLSFS
GLLTEAIRKYRSGKYRVEDLAYSFQETAFAALVEVTERAVAHTEKDEVVL
VGGVAANNRLREMLRIMTEDRGIKFFVPPYDLCRDNGAMIAYTGLRMYKA
GISFRLEETIVKQKFRTDEVEIVWH

3D structure

• PDB: 2ivp An Archaeal Orthologue of the Universal Protein Kae1 is an Iron Metalloprotein which Exhibits Atypical DNA-Binding Properties and Apurinic-Endonuclease Activity in Vitro.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.3.1.234: N(6)-L-threonylcarbamoyladenine synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE2	A	H107 H111 Y127 D285	H107 H111 Y127 D285
BS02	ATP	A	S129 G130 G131 G155 D159 G172 P173 G253 A256 N257 R284 D285	S129 G130 G131 G155 D159 G172 P173 G253 A256 N257 R284 D285

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003727 single-stranded RNA binding
• GO:0005506 iron ion binding
• GO:0016746 acyltransferase activity
• GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups
• GO:0046872 metal ion binding
• GO:0061711 N(6)-L-threonylcarbamoyladenine synthase activity

Biological Process

• GO:0002949 tRNA threonylcarbamoyladenosine modification
• GO:0006400 tRNA modification
• GO:0008033 tRNA processing
• GO:0070525 tRNA threonylcarbamoyladenosine metabolic process

Cellular Component

• GO:0000408 EKC/KEOPS complex
• GO:0005737 cytoplasm

External links

• PDB: RCSB:2ivp, PDBe:2ivp, PDBj:2ivp
• PDBsum: 2ivp
• PubMed: 17766251
• UniProt: Q9UXT7|KAE1_PYRAB tRNA N6-adenosine threonylcarbamoyltransferase (Gene Name=kae1)