Structure of PDB 2ivh Chain A

Receptor sequence

>2ivhA (length=124) Species: 562 (Escherichia coli)

KPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKK
FWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELHQEKP
ISVYDMDNISVVTPKRHIDIHRGK

3D structure

• PDB: 2ivh Structural Basis for Sequence-Dependent DNA Cleavage by Nonspecific Endonucleases.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R538 E542 H544 Q545 H569 H573
• Catalytic site (residue number reindexed from 1): R90 E94 H96 Q97 H117 H121
• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R496 K525 S540 E542 L543 H544 H569 H573	R48 K77 S92 E94 L95 H96 H117 H121
BS02	dna	A	K490 G536 K537 R538 I570 R574	K42 G88 K89 R90 I118 R122
BS03	ZN	A	H544 H569 H573	H96 H117 H121

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity
• GO:0005102 signaling receptor binding

Biological Process

• GO:0009617 response to bacterium
• GO:0019835 cytolysis

External links

• PDB: RCSB:2ivh, PDBe:2ivh, PDBj:2ivh
• PDBsum: 2ivh
• PubMed: 17175542
• UniProt: Q47112|CEA7_ECOLX Colicin-E7 (Gene Name=colE7)