Structure of PDB 2itb Chain A

Receptor sequence
>2itbA (length=199) Species: 160488 (Pseudomonas putida KT2440) [Search protein sequence]
LIPEIDAFLGCPTPDAWIEAALADQETLLIDHKNCEFKAASTALSLIAKY
NTHLDLINMMSRLAREELVHHEQVLRLMKRRGVPLRPVSAGRYASGLRRL
VRAHEPVKLVDTLVVGAFIEARSCERFAALVPHLDEELGRFYHGLLKSEA
RHYQGYLKLAHNYGDEADIARCVELVRAAEMELIQSPDQELRFHSGIPQ
3D structure
PDB2itb Crystal structure of putative tRNA-(ms(2)io(6)a)-hydroxylase (NP_744337.1) from Pseudomonas Putida KT2440 at 2.05 A resolution
ChainA
Resolution2.05 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.14.99.69: tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE A E38 E69 H72 E151 E36 E67 H70 E149
BS02 FE A E69 E122 E151 H154 E67 E120 E149 H152
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0045301 tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine)-hydroxylase activity
GO:0046872 metal ion binding
Biological Process
GO:0006400 tRNA modification
GO:0008033 tRNA processing

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Molecular Function
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Biological Process
External links
PDB RCSB:2itb, PDBe:2itb, PDBj:2itb
PDBsum2itb
PubMed
UniProtQ88KV1|MIAE_PSEPK tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase (Gene Name=miaE)

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