Structure of PDB 2is4 Chain A

Receptor sequence
>2is4A (length=646) Species: 562 (Escherichia coli) [Search protein sequence]
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVE
NCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRA
HHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQK
DEGLRPHHIQNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNK
PHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSI
YGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGR
LGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAIL
YRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIVNRND
DAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGR
AASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQ
TRIENLEELVTATRQFMPLQAFLSHAALEAGETWQDAVQLMTLHSAKGLE
FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAE
TRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTP
3D structure
PDB2is4 UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.
ChainA
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T36 D220 Q251
Catalytic site (residue number reindexed from 1) T36 D217 Q248
Enzyme Commision number 5.6.2.4: DNA 3'-5' helicase.
Interaction with ligand
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0015616 DNA translocase activity
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0042803 protein homodimerization activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000717 nucleotide-excision repair, DNA duplex unwinding
GO:0000725 recombinational repair
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006281 DNA repair
GO:0006289 nucleotide-excision repair
GO:0006298 mismatch repair
GO:0009314 response to radiation
GO:0009432 SOS response
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
GO:0070581 rolling circle DNA replication
GO:0070716 mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0017117 single-stranded DNA-dependent ATP-dependent DNA helicase complex
GO:0033202 DNA helicase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2is4, PDBe:2is4, PDBj:2is4
PDBsum2is4
PubMed17190599
UniProtP03018|UVRD_ECOLI DNA helicase II (Gene Name=uvrD)

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