Structure of PDB 2is2 Chain A

Receptor sequence
>2is2A (length=642) Species: 562 (Escherichia coli) [Search protein sequence]
DVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVEN
CSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAH
HMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKD
EGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWL
NKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQ
SIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNN
GRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECA
ILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIVNR
NDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALA
GRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEK
GQTRIENLEELVTATRQFSYDEDLMPLQAFLSHAALEAGEGQADTWQDAV
QLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVT
RAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRL
3D structure
PDB2is2 UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.
ChainA
Resolution3.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T36 D220 Q251
Catalytic site (residue number reindexed from 1) T35 D219 Q250
Enzyme Commision number 5.6.2.4: DNA 3'-5' helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T63 N64 H91 F189 W256 R257 R355 N357 R381 N412 G417 G419 R421 T422 L540 T558 H560 M584 Y621 T62 N63 H90 F188 W255 R256 R354 N356 R380 N411 G416 G418 R420 T421 L536 T554 H556 M580 Y617
BS02 dna A K124 R453 Y621 G622 K123 R452 Y617 G618
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0015616 DNA translocase activity
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0042803 protein homodimerization activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000717 nucleotide-excision repair, DNA duplex unwinding
GO:0000725 recombinational repair
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006281 DNA repair
GO:0006289 nucleotide-excision repair
GO:0006298 mismatch repair
GO:0009314 response to radiation
GO:0009432 SOS response
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
GO:0070581 rolling circle DNA replication
GO:0070716 mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0017117 single-stranded DNA-dependent ATP-dependent DNA helicase complex
GO:0033202 DNA helicase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2is2, PDBe:2is2, PDBj:2is2
PDBsum2is2
PubMed17190599
UniProtP03018|UVRD_ECOLI DNA helicase II (Gene Name=uvrD)

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