Structure of PDB 2ipw Chain A

Receptor sequence
>2ipwA (length=309) Species: 9606 (Homo sapiens) [Search protein sequence]
SRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNE
NEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKL
DYLDLYLIHWPTGFKPGKEFFPLDNVVPSDTNILDTWAAMEELVDEGLVK
AIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGI
VVTAYSPLGSPDRPYAEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRN
LVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVAALLSCTSH
KDYPFHEEF
3D structure
PDB2ipw Structural and thermodynamic studies of simple aldose reductase-inhibitor complexes.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D43 Y48 K77 H110
Catalytic site (residue number reindexed from 1) D42 Y47 K76 H109
Enzyme Commision number 1.1.1.21: aldose reductase.
1.1.1.300: NADP-retinol dehydrogenase.
1.1.1.372: D/L-glyceraldehyde reductase.
1.1.1.54: allyl-alcohol dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP A G18 T19 W20 K21 D43 Y48 H110 Q183 Y209 S210 P211 L212 S214 P215 D216 A245 I260 P261 K262 S263 V264 T265 R268 E271 N272 G17 T18 W19 K20 D42 Y47 H109 Q179 Y205 S206 P207 L208 S210 P211 D212 A239 I254 P255 K256 S257 V258 T259 R262 E265 N266
BS02 2CL A W20 Y48 H110 W19 Y47 H109 MOAD: Ki=1uM
BindingDB: Ki=4400nM,IC50=4400nM
Gene Ontology
Molecular Function
GO:0001758 retinal dehydrogenase activity
GO:0004032 aldose reductase (NADPH) activity
GO:0005515 protein binding
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0036130 prostaglandin H2 endoperoxidase reductase activity
GO:0043795 glyceraldehyde oxidoreductase activity
GO:0047655 allyl-alcohol dehydrogenase activity
GO:0047939 L-glucuronate reductase activity
GO:0047956 glycerol dehydrogenase (NADP+) activity
GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity
Biological Process
GO:0001523 retinoid metabolic process
GO:0002070 epithelial cell maturation
GO:0003091 renal water homeostasis
GO:0005975 carbohydrate metabolic process
GO:0006629 lipid metabolic process
GO:0006693 prostaglandin metabolic process
GO:0006700 C21-steroid hormone biosynthetic process
GO:0019853 L-ascorbic acid biosynthetic process
GO:0035809 regulation of urine volume
GO:0042572 retinol metabolic process
GO:0043066 negative regulation of apoptotic process
GO:0044597 daunorubicin metabolic process
GO:0044598 doxorubicin metabolic process
GO:0046370 fructose biosynthetic process
GO:0071475 cellular hyperosmotic salinity response
GO:0072205 metanephric collecting duct development
Cellular Component
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0070062 extracellular exosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2ipw, PDBe:2ipw, PDBj:2ipw
PDBsum2ipw
PubMed17083960
UniProtP15121|ALDR_HUMAN Aldo-keto reductase family 1 member B1 (Gene Name=AKR1B1)

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