Structure of PDB 2io7 Chain A

Receptor sequence
>2io7A (length=587) Species: 562 (Escherichia coli) [Search protein sequence]
APFGTLLGYAPGGVAIYSSDYRSYIDDEYMGHKWQCVEFARRFLFLNYGV
VFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKG
GEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGC
YTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFD
GKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLM
YLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDE
RGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAW
KHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELGWDA
AGQLIDGEGRLVNCVWKTWAWETAFDQIREFAAVPIRTGHPQNEVRLIDV
LLRPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKT
GYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDG
KYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLI
3D structure
PDB2io7 Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase
ChainA
Resolution2.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.1.78: glutathionylspermidine amidase.
6.3.1.8: glutathionylspermidine synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A E330 N332 E307 N309
BS02 MG A D318 E330 D295 E307
BS03 ANP A D318 Y329 E330 N332 K498 A531 K533 R538 C539 G540 Q568 Q569 L570 W571 C572 Q582 L603 V604 I605 D295 Y306 E307 N309 K470 A503 K505 R510 C511 G512 Q540 Q541 L542 W543 C544 Q554 L575 V576 I577
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008884 glutathionylspermidine amidase activity
GO:0008885 glutathionylspermidine synthase activity
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0008216 spermidine metabolic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2io7, PDBe:2io7, PDBj:2io7
PDBsum2io7
PubMed17124497
UniProtP0AES0|GSP_ECOLI Bifunctional glutathionylspermidine synthetase/amidase (Gene Name=gss)

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