Structure of PDB 2id4 Chain A

Receptor sequence

>2id4A (length=480) Species: 4932 (Saccharomyces cerevisiae)

LPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLWYNNITGAGVVA
AIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCAGEI
AAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSC
SWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDN
CNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSS
DINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNLTWRDVQYLSILSAVGL
EKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEMSKTWENVNAQTWFYLP
TLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHVTVTVDIDTEIRGT
TTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVGDWKIK
VKTTENGHRIDFHSWRLKLFGESIDSSKTE

3D structure

• PDB: 2id4 Differential P1 arginine and lysine recognition in the prototypical proprotein convertase Kex2.
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D175 H213 N314 S385
• Catalytic site (residue number reindexed from 1): D54 H92 N193 S264
• Enzyme Commision number: 3.4.21.61: kexin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	D176 D210 H213 L246 I250 E255 S272 W273 G274 P275 D277 Q283 N314 S385	D55 D89 H92 L125 I129 E134 S151 W152 G153 P154 D156 Q162 N193 S264
BS02	peptide	A	H213 S385	H92 S264
BS03	CA	A	D277 D320 E350	D156 D199 E229
BS04	CA	A	D135 D184 K224 N227 F229 G231	D14 D63 K103 N106 F108 G110

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:2id4, PDBe:2id4, PDBj:2id4
• PDBsum: 2id4
• PubMed: 17426142
• UniProt: P13134|KEX2_YEAST Kexin (Gene Name=KEX2)