Structure of PDB 2hw3 Chain A

Receptor sequence

>2hw3A (length=580) Species: 1422 (Geobacillus stearothermophilus)

KKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

3D structure

• PDB: 2hw3 The structural basis for the mutagenicity of O6-methyl-guanine lesions.
• Chain: A
• Resolution: 1.98 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T550 K551 T552 T556 S557 A558 R578 K582 Y587 R615 Q624 N625 I626 P627 I628 R629 V828 H829 D830	T254 K255 T256 T260 S261 A262 R282 K286 Y291 R319 Q328 N329 I330 P331 I332 R333 V532 H533 D534
BS02	dna	A	N527 N529 S530 S585 T586 L610 T611 Q612 T613 S617 S618 T619 N622 N625 G711 Y714 S717 G720 L721 N724 R771 F786 R789 M790 N793 Q797	N231 N233 S234 S289 T290 L314 T315 Q316 T317 S321 S322 T323 N326 N329 G415 Y418 S421 G424 L425 N428 R475 F490 R493 M494 N497 Q501
BS03	GLC	A	E325 R375 I605	E29 R79 I309
BS04	FRU	A	D372 R375 D408 N607	D76 R79 D112 N311
BS05	GLC	A	Y519 N527 I528 N529 Y577	Y223 N231 I232 N233 Y281
BS06	FRU	A	F526 N527	F230 N231
BS07	MG	A	Y654 D830	Y358 D534

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:2hw3, PDBe:2hw3, PDBj:2hw3
• PDBsum: 2hw3
• PubMed: 17179038
• UniProt: Q45458