Structure of PDB 2hvi Chain A

Receptor sequence

>2hviA (length=580) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

KKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNYGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

3D structure

PDB

2hvi The structural basis for the mutagenicity of O6-methyl-guanine lesions.

Chain

Resolution

1.98 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T550 K551 T552 T556 S557 A558 R578 K582 Y587 R615 Q624 N625 I626 P627 I628 R629 H829	T254 K255 T256 T260 S261 A262 R282 K286 Y291 R319 Q328 N329 I330 P331 I332 R333 H533	PDBbind-CN: Kd=17.9uM
BS02	dna	A	N527 N529 S530 S585 T586 T611 Q612 T613 S617 S618 T619 E620 N622 G711 Y714 I716 S717 Y719 G720 R771 F781 N782 F786 R789 N793 Q797	N231 N233 S234 S289 T290 T315 Q316 T317 S321 S322 T323 E324 N326 G415 Y418 I420 S421 Y423 G424 R475 F485 N486 F490 R493 N497 Q501	PDBbind-CN: Kd=17.9uM
BS03	GLC	A	E325 I605	E29 I309
BS04	FRU	A	Y327 D372 R375 D408 N607	Y31 D76 R79 D112 N311
BS05	DCT	A	R615 Y654 S655 Q656 I657 E658 H682 R702 K706 Y710 D830	R319 Y358 S359 Q360 I361 E362 H386 R406 K410 Y414 D534
BS06	DCT	A	E469 Q470 D471 R472 L473 L767 H768	E173 Q174 D175 R176 L177 L471 H472
BS07	MG	A	D653 Y654 D830	D357 Y358 D534

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0008408	3'-5' exonuclease activity

	Biological Process
GO:0006139	nucleobase-containing compound metabolic process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:2hvi, PDBe:2hvi, PDBj:2hvi
PDBsum	2hvi
PubMed	17179038
UniProt	Q5KWC1

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