Structure of PDB 2hqm Chain A

Receptor sequence

>2hqmA (length=461) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

KHYDYLVIGGGSGGVASARRAASYGAKTLLVEAKALGGTCVNVGCVPKKV
MWYASDLATRVSHANEYGLYQNLPLDKEHLTFNWPEFKQKRDAYVHRLNG
IYQKNLEKEKVDVVFGWARFNKDGNVEVQKRDNTTEVYSANHILVATGGK
AIFPENIPGFELGTDSDGFFRLEEQPKKVVVVGAGYIGIELAGVFHGLGS
ETHLVIRGETVLRKFDECIQNTITDHYVKEGINVHKLSKIVKVEKNVTDK
LKIHMNDSKSIDDVDELIWTIGRKSHLGMGSENVGIKLNSHDQIIADEYQ
NTNVPNIYSLGDVVGKVELTPVAIAAGRKLSNRLFGPEKFRNDKLDYENV
PSVIFSHPEAGSIGISEKEAIEKYGKENIKVYNSKFTAMYYAMLSEKSPT
RYKIVCAGPNEKVVGLHIVGDSSAEILQGFGVAIKMGATKADFDNCVAIH
PTSAEELVTMR

3D structure

PDB

2hqm Crystal structure of glutathione reductase Glr1 from the yeast Saccharomyces cerevisiae.

Chain

Resolution

2.4 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	L57 C61 C66 K69 Y207 E211 A470 H472 E477
Catalytic site (residue number reindexed from 1)	L36 C40 C45 K48 Y186 E190 A448 H450 E455
Enzyme Commision number	1.8.1.7: glutathione-disulfide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	G30 G32 S33 G34 E53 A54 G59 T60 C61 G65 C66 K69 W138 A139 T168 G169 Y207 I208 R295 H298 G333 D334 E340 L341 T342	G9 G11 S12 G13 E32 A33 G38 T39 C40 G44 C45 K48 W117 A118 T147 G148 Y186 I187 R273 H276 G311 D312 E318 L319 T320
BS02	GSH	A	E238 C239 N242 N405 R423 K425	E217 C218 N221 N383 R401 K403

Gene Ontology

	Molecular Function
GO:0004362	glutathione-disulfide reductase (NADPH) activity
GO:0016491	oxidoreductase activity
GO:0016668	oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

	Biological Process
GO:0006749	glutathione metabolic process
GO:0034599	cellular response to oxidative stress
GO:0045454	cell redox homeostasis
GO:0098869	cellular oxidant detoxification

	Cellular Component
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005777	peroxisome
GO:0005829	cytosol

View graph for
Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:2hqm, PDBe:2hqm, PDBj:2hqm
PDBsum	2hqm
PubMed	17554778
UniProt	P41921\|GSHR_YEAST Glutathione reductase (Gene Name=GLR1)

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