Structure of PDB 2hke Chain A

Receptor sequence

>2hkeA (length=377) Species: 5691 (Trypanosoma brucei)

QCVTVEAPINIAFIKYWGKREGGETLILPTNDSFSITLSASPFRSKTSVE
LRDDIETDTLRLNGTEVDVGKTPRVQSMLLHLRSTCPEELKNKKVNIVSE
NNFPTAAGMASSASGYCAMSAALIRAFKSTTNVSMLARLGSGSACRSAFG
GFVIWNKGEKPDGSDCVATQFVDETHWPEIQVMCAVLKGAQKDVSSTKGM
QQSLKTSPLMKKRISETVPERMKIASRAIKARDFATFAEIAMLESDDLQE
ICATTEPKITYATEDSYAMIRLVKAYNAKKGRTALAYTFDAGANCFLFVL
KEDLPEAVAMLMEHFPTPFEKFFFGDRELLEKVKVVSLPDEYKKLIDHPK
KPFEMLLQSPVGCGVKYLGPSESLIPP

3D structure

• PDB: 2hke Crystal Structures of Trypanosoma brucei and Staphylococcus aureus Mevalonate Diphosphate Decarboxylase Inform on the Determinants of Specificity and Reactivity
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 4.1.1.33: diphosphomevalonate decarboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SO4	A	Y19 K22 R77 S144 G145 T200	Y16 K19 R74 S141 G142 T197
BS02	SO4	A	R274 I349 D350	R271 I346 D347
BS03	SO4	A	S42 A43 G365 C366	S39 A40 G362 C363

Gene Ontology

Molecular Function

• GO:0004163 diphosphomevalonate decarboxylase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0016829 lyase activity
• GO:0016831 carboxy-lyase activity

Biological Process

• GO:0006695 cholesterol biosynthetic process
• GO:0008299 isoprenoid biosynthetic process
• GO:0016310 phosphorylation
• GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005929 cilium

External links

• PDB: RCSB:2hke, PDBe:2hke, PDBj:2hke
• PDBsum: 2hke
• PubMed: 17583736
• UniProt: Q388P2