Structure of PDB 2h5e Chain A

Receptor sequence
>2h5eA (length=488) Species: 562 (Escherichia coli) [Search protein sequence]
LSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQITTSVMQFPYHDCL
VNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLR
DTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFKGVY
HLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELEL
VKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQ
TDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQ
VRTAKDVVISDALTFMAVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFT
GIPNFAPELFRRIRLKDPKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAV
GVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNES
QLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
3D structure
PDB2h5e RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors
ChainA
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) D23
Catalytic site (residue number reindexed from 1) D21
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GDP A D23 G25 K26 T27 T28 H92 N142 K143 D145 T258 L260 D21 G23 K24 T25 T26 H59 N109 K110 D112 T225 L227 PDBbind-CN: -logKd/Ki=8.25,Kd=5.6nM
Gene Ontology
Molecular Function
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0016149 translation release factor activity, codon specific
GO:0016150 translation release factor activity, codon nonspecific
GO:0019003 GDP binding
GO:0097216 guanosine tetraphosphate binding
Biological Process
GO:0006412 translation
GO:0006415 translational termination
GO:0006449 regulation of translational termination
GO:1990145 maintenance of translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:2h5e, PDBe:2h5e, PDBj:2h5e
PDBsum2h5e
PubMed17540173
UniProtP0A7I4|RF3_ECOLI Peptide chain release factor RF3 (Gene Name=prfC)

[Back to BioLiP]