Structure of PDB 2h12 Chain A

Receptor sequence
>2h12A (length=418) Species: 435 (Acetobacter aceti) [Search protein sequence]
STATISVDGKSAEMPVLSGTLGPDVIDIRKLPAQLGVFTFDPGYGETAAC
NSKITFIDGDKGVLLHRGYPIAQLAENASYEEVIYLLLNGELPNKAQYDT
FTNTLTNHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYPANRDLA
AMRLIAKIPTIAAWAYKYTQGEAFIYPRNDLNYAENFLSMMFARMSEPYK
VNPVLARAMNRILILHADHEQNASTSTVRLAGSTGANPFACIAAGIAALW
GPAHGGANEAVLKMLARIGKKENIPAFIAQVKDKNSGVKLMGFGHRVYKN
FDPRAKIMQQTCHEVLTELGIKDDPLLDLAVELEKIALSDDYFVQRKLYP
NVDFYSGIILKAMGIPTSMFTVLFAVARTTGWVSQWKEMIEEPGQRISRP
RQLYIGAPQRDYVPLAKR
3D structure
PDB2h12 Structure of a NADH-insensitive hexameric citrate synthase that resists acid inactivation.
ChainA
Resolution1.85 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) S242 H272 H313 R322 D371
Catalytic site (residue number reindexed from 1) S224 H254 H295 R304 D353
Enzyme Commision number 2.3.3.16: citrate synthase (unknown stereospecificity).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 OAA A H237 N240 H313 R322 R396 H219 N222 H295 R304 R378 MOAD: Kd=21.1uM
BS02 CMX A A271 H272 A275 K307 L308 G310 F311 G312 R314 Y360 R364 L366 N369 D371 A253 H254 A257 K289 L290 G292 F293 G294 R296 Y342 R346 L348 N351 D353
Gene Ontology
Molecular Function
GO:0004108 citrate (Si)-synthase activity
GO:0016740 transferase activity
GO:0036440 citrate synthase activity
GO:0046912 acyltransferase activity, acyl groups converted into alkyl on transfer
Biological Process
GO:0006099 tricarboxylic acid cycle
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:2h12, PDBe:2h12, PDBj:2h12
PDBsum2h12
PubMed17087502
UniProtP20901|CISY_ACEAC Citrate synthase (Gene Name=aarA)

[Back to BioLiP]