Structure of PDB 2gws Chain A

Receptor sequence

>2gwsA (length=327) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPG
RVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 2gws Promiscuous mismatch extension by human DNA polymerase lambda.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 T371 E465 E466 N467 Y505 R517 K521	W26 T123 E217 E218 N219 Y257 R269 K273
BS02	dna	A	W342 G343 A344 G345 K347 T348 K472	W94 G95 A96 G97 K99 T100 K224
BS03	dna	A	Y267 W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312 K324	Y19 W26 R27 G30 Y31 P55 G56 G58 R60 M61 K64 K76
BS04	dna	A	V537 R549	V289 R301

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2gws, PDBe:2gws, PDBj:2gws
• PDBsum: 2gws
• PubMed: 16807316
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)