Structure of PDB 2gmh Chain A

Receptor sequence

>2gmhA (length=581) Species: 9823 (Sus scrofa)

CKVPRITTHYTIYPRDQDKRWEGVNMERFAEEADVVIVGAGPAGLSAATR
LKQLAAQHEKDLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGA
PLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYVVRLGHLVSWMGEQ
AEALGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLE
LHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKW
KPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSP
FREFQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIG
CSPGFMNVPKIKGTHTAMKSGTLAAESIFNQLTSENLQSKTIGLHVTEYE
DNLKNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTL
KHKGSDSDQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAH
LTLKDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNC
VHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

3D structure

• PDB: 2gmh Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S82 R331 T367 C528 C553 C556 C559
• Catalytic site (residue number reindexed from 1): S79 R328 T364 C525 C550 C553 C556
• Enzyme Commision number: 1.5.5.1: electron-transferring-flavoprotein dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	L504 C528 P529 V532 C553 V554 C556 K557 T558 C559 W570	L501 C525 P526 V529 C550 V551 C553 K554 T555 C556 W567
BS02	FAD	A	V41 G42 P45 A46 E71 K72 H79 S82 G83 A84 C85 A166 A167 A212 E213 G214 R331 G353 C354 I364 K365 G366 T367	V38 G39 P42 A43 E68 K69 H76 S79 G80 A81 C82 A163 A164 A209 E210 G211 R328 G350 C351 I361 K362 G363 T364
BS03	UQ5	A	F114 M133 H260 V262 Y271 G272 G273 S274 I364 G434 M435 T438 G439	F111 M130 H257 V259 Y268 G269 G270 S271 I361 G431 M432 T435 G436

Gene Ontology

Molecular Function

• GO:0004174 electron-transferring-flavoprotein dehydrogenase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0048039 ubiquinone binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0022900 electron transport chain
• GO:0022904 respiratory electron transport chain

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0016020 membrane

External links

• PDB: RCSB:2gmh, PDBe:2gmh, PDBj:2gmh
• PDBsum: 2gmh
• PubMed: 17050691
• UniProt: P55931|ETFD_PIG Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (Gene Name=ETFDH)