Structure of PDB 2gih Chain A

Receptor sequence
>2gihA (length=257) Species: 727 (Haemophilus influenzae) [Search protein sequence]
SFIKPIYQDINSILIGQKVKRPKSGTLSGHAAGEPFEKLVYKFLKENLSD
LTFKQYEYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSI
ENLFEEKQNDTADILLVKDQFYELLDVKTRNISKSAFAPNIISAYKLAQT
CAKMIDNKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYIN
WAAAMQIQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVK
PFKKYIL
3D structure
PDB2gih Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
ChainA
Resolution2.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A R91 K93 K108 Q109 F138 Y199 A203 A204 R241 K248 R90 K92 K107 Q108 F137 Y198 A202 A203 R240 K247 PDBbind-CN: Kd=1.03nM
BS02 dna A G30 H31 Q109 N110 A137 F138 A139 N141 S144 K147 A205 M206 Q207 G29 H30 Q108 N109 A136 F137 A138 N140 S143 K146 A204 M205 Q206 PDBbind-CN: Kd=1.03nM
BS03 CA A D114 D127 V128 D113 D126 V127
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2gih, PDBe:2gih, PDBj:2gih
PDBsum2gih
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

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