Structure of PDB 2gep Chain A

Receptor sequence

>2gepA (length=472) Species: 37762 (Escherichia coli B) [Search protein sequence]

LLRCRLPGGVITTKQWQAIDKFAGENTIYGSIRLTNRQTFQFHGILKKNV
KPVHQMLHSVGLDALATANDMNRNVLCTSNPYESQLHAEAYEWAKKISEH
LLPRTEPILGQTYLPRKFKTTVVIPPQNDIDLHANDMNFVAIAENGKLVG
FNLLVGGGLSIEHGNKKTYARTASEFGYLPLEHTLAVAEAVVTTQRDWGN
RTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPIRPYEFTGRGDRIGW
VKGIDDNWHLTLFIENGRILDYPARPLKTGLLEIAKIHKGDFRITANQNL
IIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAE
RFLPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAP
GRYNLHLGGNRIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGF
GDFTVRAGIIRPVLDPARDLWD

3D structure

PDB

2gep Probing the catalytic mechanism of sulfite reductase by X-ray crystallography: structures of the Escherichia coli hemoprotein in complex with substrates, inhibitors, intermediates, and products.

Chain

Resolution

1.9 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R83 R153 K215 K217 A394 C434 C440 C479 C483
Catalytic site (residue number reindexed from 1)	R3 R73 K117 K119 A296 C336 C342 C381 C385
Enzyme Commision number	1.8.1.2: assimilatory sulfite reductase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	SO3	A	R83 R153 K215 K217	R3 R73 K117 K119
BS02	SF4	A	C434 S436 C440 A443 G478 C479 N481 C483	C336 S338 C342 A345 G380 C381 N383 C385
BS03	SRM	A	L81 R83 R113 T115 N116 R117 Q121 H123 R214 K215 K217 G256 L257 S258 Q396 C434 V435 T439 C440 L442 N481 G482 C483 R485	L1 R3 R33 T35 N36 R37 Q41 H43 R116 K117 K119 G158 L159 S160 Q298 C336 V337 T341 C342 L344 N383 G384 C385 R387

Gene Ontology

	Molecular Function
GO:0004783	sulfite reductase (NADPH) activity
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0050661	NADP binding
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:0008652	amino acid biosynthetic process

	Cellular Component
GO:0009337	sulfite reductase complex (NADPH)

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:2gep, PDBe:2gep, PDBj:2gep
PDBsum	2gep
PubMed	9315849
UniProt	P17846\|CYSI_ECOLI Sulfite reductase [NADPH] hemoprotein beta-component (Gene Name=cysI)

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