Structure of PDB 2flc Chain A

Receptor sequence

>2flcA (length=247) Species: 727 (Haemophilus influenzae) [Search protein sequence]

MNLVELGSKTAKDGFKNEKDIADRFENWKENSEAQDWLVTMGHNLDEIKS
VKAVVLSGYKSDINVQVLVFYKDALDIHNIQVKLVSNKRGFNQIDKHWLA
HYQEMWKFDDNLLRILRHFTGELPPYHSNTKDKRRMFMTEFSQEEQNIVL
NWLEKNRVLVLTDILRGRGDFAAEWVLVAQKVSNNARWILRNINEVLQHY
GSGDISLSPRGSINFGRVTIQRKGGDNGRETANMLQFKIDPTELFDI

3D structure

PDB

2flc DNA nicking by HinP1I endonuclease: bending, base flipping and minor groove expansion.

Chain

Resolution

2.59 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	G58 Y59 Q93 K96 H97	G58 Y59 Q93 K96 H97
BS02	dna	A	T10 A11 Q81 K83 L84 S86 F91 N92 Q93 R210 K238	T10 A11 Q81 K83 L84 S86 F91 N92 Q93 R210 K238
BS03	dna	A	L3 V4 G7 S8 S57 F91 R134 S208 R210 Q221 K223 G224 G225 D226 N227 R229 K238	L3 V4 G7 S8 S57 F91 R134 S208 R210 Q221 K223 G224 G225 D226 N227 R229 K238
BS04	MG	A	D62 Q81 V82	D62 Q81 V82

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

View graph for
Molecular Function

External links

PDB	RCSB:2flc, PDBe:2flc, PDBj:2flc
PDBsum	2flc
PubMed	16473850
UniProt	Q5I6E6

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