Structure of PDB 2fkc Chain A

Receptor sequence

>2fkcA (length=247) Species: 727 (Haemophilus influenzae)

MNLVELGSKTAKDGFKNEKDIADRFENWKENSEAQDWLVTMGHNLDEIKS
VKAVVLSGYKSDINVQVLVFYKDALDIHNIQVKLVSNKRGFNQIDKHWLA
HYQEMWKFDDNLLRILRHFTGELPPYHSNTKDKRRMFMTEFSQEEQNIVL
NWLEKNRVLVLTDILRGRGDFAAEWVLVAQKVSNNARWILRNINEVLQHY
GSGDISLSPRGSINFGRVTIQRKGGDNGRETANMLQFKIDPTELFDI

3D structure

• PDB: 2fkc DNA nicking by HinP1I endonuclease: bending, base flipping and minor groove expansion.
• Chain: A
• Resolution: 2.39 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T10 A11 G14 F15 L84 V85 S86 N87 F91 N92 Q93 K96 Q236 K238	T10 A11 G14 F15 L84 V85 S86 N87 F91 N92 Q93 K96 Q236 K238
BS02	dna	A	L3 V4 G7 S8 A11 K12 F15 F91 R134 S208 R210 Q221 K223 G224 G225 D226 N227 K238	L3 V4 G7 S8 A11 K12 F15 F91 R134 S208 R210 Q221 K223 G224 G225 D226 N227 K238
BS03	CA	A	E18 D62	E18 D62
BS04	CA	A	D62 Q81 V82	D62 Q81 V82

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

External links

• PDB: RCSB:2fkc, PDBe:2fkc, PDBj:2fkc
• PDBsum: 2fkc
• PubMed: 16473850
• UniProt: Q5I6E6