Structure of PDB 2fbw Chain A

Receptor sequence

>2fbwA (length=612) Species: 9031 (Gallus gallus) [Search protein sequence]

TQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVA
AQGGINAALGNMEDDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPAAVIE
LENYGMPFSRTEEGKIYQRAFGGQSLQFGKGGQAHRCCCVADRTGHSLLH
TLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGTIHRFRAKNT
VIATGGYGRTYFSCTSAHTSTGDGTAMVTRAGLPCQDLEFVQFHPTGIYG
AGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTIEIR
EGRGCGPEKDHVYLQLHHLPPQQLATRLPGISETAMIFAGVDVTKEPIPV
LPTVHYNMGGIPTNYKGQVITHVNGEDKVVPGLYACGEAASASVHGANRL
GANSLLDLVVFGRACALTIAETCKPGEPVPSIKPNAGEESVANLDKLRFA
DGTIRTSEARLNMQKTMQSHAAVFRTGSILQEGCEKLSQIYRDLAHLKTF
DRGIVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKLRID
EFDYSKPLQGQQKRPFEEHWRKHTLSYVDVKSGKVTLKYRPVIDRTLNEE
DCSSVPPAIRSY

3D structure

PDB

2fbw 3-nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme.

Chain

Resolution

2.06 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	F130 Q251 H253 L263 E266 R269 R297 H364 Y365 R408
Catalytic site (residue number reindexed from 1)	F121 Q242 H244 L254 E257 R260 R288 H355 Y356 R399
Enzyme Commision number	1.3.5.1: succinate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	G25 A26 G27 A29 T48 K49 S55 H56 T57 A60 Q61 G62 G63 F177 A178 A212 T213 D232 L263 H364 Y365 G396 E397 A411 S413 L414 L417	G16 A17 G18 A20 T39 K40 S46 H47 T48 A51 Q52 G53 G54 F168 A169 A203 T204 D223 L254 H355 Y356 G387 E388 A402 S404 L405 L408
BS02	Y3P	A	G62 F130 H253 L263 T265 E266 R297 H364 R408 G410 A411	G53 F121 H244 L254 T256 E257 R288 H355 R399 G401 A402

Gene Ontology

	Molecular Function
GO:0008177	succinate dehydrogenase (quinone) activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors
GO:0050660	flavin adenine dinucleotide binding

	Biological Process
GO:0006099	tricarboxylic acid cycle
GO:0006105	succinate metabolic process
GO:0006121	mitochondrial electron transport, succinate to ubiquinone
GO:0022900	electron transport chain
GO:0022904	respiratory electron transport chain

	Cellular Component
GO:0005739	mitochondrion
GO:0005743	mitochondrial inner membrane
GO:0045273	respiratory chain complex II (succinate dehydrogenase)

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2fbw, PDBe:2fbw, PDBj:2fbw
PDBsum	2fbw
PubMed	16371358
UniProt	Q9YHT1\|SDHA_CHICK Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (Gene Name=SDHA)

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