Structure of PDB 2fah Chain A

Receptor sequence

>2fahA (length=608) Species: 9031 (Gallus gallus)

LSTSLSALPAAARDFVEEAVRLCRPREVLLCDGSEEEGKELLRGLQDDGV
LHPLPKYDNCWLARTDPRDVARVESKTVLVTPEQSDAVPPPPPSGGPPQL
GNWMSPNAFQAAVQERFPGCMAGRPLYVIPFSMGPPTSPLAKLGVQVTDS
PYVVLSMRIMTRVGPAVLQRLDDDFVRCLHSVGRPLPLTEPLVSSWPCDP
SRVLVAHIPSERRIVSFGSGYGGNSLLGKKCFALRIASRMAQQQGWLAEH
MLILGVTSPSGEKRYMAAAFPSACGKTNLAMMTPSLPGWRIHCVGDDIAW
MKFDDEGRLRAINPERGFFGVAPGTSSRTNPNAMATIARNTIFTNVGLRS
DGGVYWDGLDEPTEPGVTYTSWLGKPWKHGDPEPCAHPNSRFCAPADQCP
IMDPRWDDPEGVPIDAIIFGGRRPRGVPLVVEAFGWRHGVFMGSAMRSEA
TAAAEHKGGRLMHDPFAMRPFFGYNAGRYLEHWLSTGLRSNARLPRLFHV
NWFLRDNEGRFVWPGFGHNARVLAWIFGRIQGRDTARPTPIGWVPKEGDL
DLGGLPGVDYSQLFPMEKGFWEEECRQLREYYGENFGADLPRDVMAELEG
LEERVRKM

3D structure

• PDB: 2fah Structural Insights into the Mechanism of PEPCK Catalysis
• Chain: A
• Resolution: 2.09 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R105 Y254 K263 H283 S305 C307 D330 R424
• Catalytic site (residue number reindexed from 1): R72 Y221 K230 H250 S272 C274 D297 R391
• Enzyme Commision number: 4.1.1.32: phosphoenolpyruvate carboxykinase (GTP).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GDP	A	A306 C307 G308 K309 T310 N311 P356 R455 F536 F544 F549	A273 C274 G275 K276 T277 N278 P323 R422 F503 F511 F516
BS02	MN	A	K263 H283 K309 D330	K230 H250 K276 D297
BS03	MN	A	T310 G353 V354	T277 G320 V321

Gene Ontology

Molecular Function

• GO:0004611 phosphoenolpyruvate carboxykinase activity
• GO:0004613 phosphoenolpyruvate carboxykinase (GTP) activity
• GO:0005525 GTP binding
• GO:0017076 purine nucleotide binding

Biological Process

• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:2fah, PDBe:2fah, PDBj:2fah
• PDBsum: 2fah
• PubMed: 16819824
• UniProt: P21642|PCKGM_CHICK Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (Gene Name=PCK2)