Structure of PDB 2f03 Chain A

Receptor sequence
>2f03A (length=269) Species: 68197 (Streptomyces fimbriatus) [Search protein sequence]
MHQDYRELSLDELESVEKQTLRTIVQALQQYSKEAKSIFETTAADSSGEV
IVLAEDITQYALEVAETYPINRRFAGFIDYKRVRWLPSPHGLLPQVLLVD
AKASTEKNRDTLQRSQLPMDAEFRNTSSGEVVTMEAGVIPHLMLQSANDG
VLPAVTTSIFVHFYYRELKDVEGRYRELKSIYVLSLPHARLKQRYNPDPD
TSFFGAGKHSPARGEVARIRVYFDRLKEACPWRLQELHYSADSEYTQPRW
RDLNDAGHEVTKEFLFLER
3D structure
PDB2f03 A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA.
ChainA
Resolution3.05 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A S46 R109 N125 T126 S127 A206 K208 S210 P211 A212 R213 E215 Y222 S46 R109 N125 T126 S127 A206 K208 S210 P211 A212 R213 E215 Y222
BS02 dna A I51 K102 A103 T105 Q113 Y165 K208 R218 I51 K102 A103 T105 Q113 Y165 K208 R218
BS03 CA A D79 D100 A101 D79 D100 A101
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2f03, PDBe:2f03, PDBj:2f03
PDBsum2f03
PubMed16308566
UniProtO52512|T2S1_STRFI Type II restriction enzyme SfiI (Gene Name=sfiIR)

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