Structure of PDB 2eg8 Chain A

Receptor sequence
>2eg8A (length=343) Species: 562 (Escherichia coli) [Search protein sequence]
SQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVE
AAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKL
YPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDRE
ARFIESVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHL
MFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAP
HARHRKESSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNGPQFY
GLPVNDTFIELVREEQQVAESIALTDDTLVPFLAGETVRWSVK
3D structure
PDB2eg8 Structures of Ligand-free and Inhibitor Complexes of Dihydroorotase from Escherichia coli: Implications for Loop Movement in Inhibitor Design
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) H16 H18 K102 H139 H177 D250
Catalytic site (residue number reindexed from 1) H13 H15 K99 H136 H174 D247
Enzyme Commision number 3.5.2.3: dihydroorotase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A K102 H139 H177 K99 H136 H174
BS02 ZN A H16 H18 K102 D250 H13 H15 K99 D247
BS03 FOT A R20 N44 H139 C221 L222 H254 A266 G267 R17 N41 H136 C218 L219 H251 A263 G264 MOAD: Ki=31.8uM
PDBbind-CN: -logKd/Ki=4.50,Ki=31.8uM
Gene Ontology
Molecular Function
GO:0004151 dihydroorotase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
GO:0006221 pyrimidine nucleotide biosynthetic process
GO:0019856 pyrimidine nucleobase biosynthetic process
GO:0044205 'de novo' UMP biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:2eg8, PDBe:2eg8, PDBj:2eg8
PDBsum2eg8
PubMed17550785
UniProtP05020|PYRC_ECOLI Dihydroorotase (Gene Name=pyrC)

[Back to BioLiP]