Structure of PDB 2e9m Chain A

Receptor sequence
>2e9mA (length=466) Species: 9606 (Homo sapiens) [Search protein sequence]
MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVFKNQTGD
VACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYY
NKIIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCF
STFGDRVKQWITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKA
HARSWHSYDSLFRKKQKGMVSLSLFAVWLEPADPNSVSDQEAAKRAITFH
LDLFAKPIFIDGDYPEVVKSQIASMSQKQGYPSSRLPEFTEEEKKMIKGT
ADFFAVQYYTTRLIKYQENKKGELGILQDAEIEFFPDPSWKNVDWIYVVP
WGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEYFRQTFQEL
FKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPY
TSAKEYAKIIRNNGLE
3D structure
PDB2e9m Klotho-related protein is a novel cytosolic neutral beta-glycosylceramidase.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R75 H120 E165 V168 Q307 Y309 E373
Catalytic site (residue number reindexed from 1) R75 H120 E165 V168 Q307 Y309 E373
Enzyme Commision number 3.2.1.21: beta-glucosidase.
3.2.1.45: glucosylceramidase.
3.2.1.46: galactosylceramidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GAL A Q17 H120 N164 E165 F225 Y309 W345 E373 W417 E424 W425 Q17 H120 N164 E165 F225 Y309 W345 E373 W417 E424 W425
BS02 PLM A V171 G190 F225 H250 W345 V171 G190 F225 H250 W345
Gene Ontology
Molecular Function
GO:0004336 galactosylceramidase activity
GO:0004348 glucosylceramidase activity
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0004565 beta-galactosidase activity
GO:0005515 protein binding
GO:0008422 beta-glucosidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0017042 glycosylceramidase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006629 lipid metabolic process
GO:0006680 glucosylceramide catabolic process
GO:0006683 galactosylceramide catabolic process
GO:0009313 oligosaccharide catabolic process
GO:0016139 glycoside catabolic process
GO:0046477 glycosylceramide catabolic process
GO:0046479 glycosphingolipid catabolic process
GO:0050821 protein stabilization
GO:1901805 beta-glucoside catabolic process
GO:1903017 positive regulation of exo-alpha-sialidase activity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1902494 catalytic complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2e9m, PDBe:2e9m, PDBj:2e9m
PDBsum2e9m
PubMed17595169
UniProtQ9H227|GBA3_HUMAN Cytosolic beta-glucosidase (Gene Name=GBA3)

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