Structure of PDB 2e7z Chain A

Receptor sequence

>2e7zA (length=727) Species: 29542 (Syntrophotalea acetylenica)

KKHVVCQSCDINCVVEAEVKADGKIQTKSISEPHPTTPPNSICMKSVNAD
TIRTHKDRVLYPLKNVGSKRGEQRWERISWDQALDEIAEKLKKIIAKYGP
ESLGVSQTEINQQSEYGTLRRFMNLLGSPNWTSAMYMCIGNTAGVHRVTH
GSYSFASFADSNCLLFIGKNLSNHNWVSQFNDLKAALKRGCKLIVLDPRR
TKVAEMADIWLPLRYGTDAALFLGMINVIINEQLYDKEFVENWCVGFEEL
KERVQEYPLDKVAEITGCDAGEIRKAAVMFATESPASIPWAVSTDMQKNS
CSAIRAQCILRAIVGSFVNGAEILGAPHSDLVPISKIQMHEALPEEKKKL
QLGTETYPFLTYTGMSALEEPSERVYGVKYFHNMGAFMANPTALFTAMAT
EKPYPVKAFFALASNALMGYANQQNALKGLMNQDLVVCYDQFMTPTAQLA
DYVLPGDHWLERPVVQPNWEGIPFGNTSQQVVEPAGEAKDEYYFIRELAV
RMGLEEHFPWKDRLELINYRISPTGMEWEEYQKQYTYMSKLPDYFGPEGV
GVATPSGKVELYSSVFEKLGYDPLPYYHEPLQTEISDPELAKEYPLILFA
GLREDSNFQSCYHQPGILRDAEPDPVALLHPKTAQSLGLPSGEWIWVETT
HGRLKLLLKHDGAQPEGTIRIPHGRWCPEQEGGPETGFSGAMLHNDAMVL
SDDDWNLDPEQGLPNLRGGILAKAYKC

3D structure

• PDB: 2e7z Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase
• Chain: A
• Resolution: 1.26 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K48 T111 I113 W134 C141 I142 V295 S338 K339 I340
• Catalytic site (residue number reindexed from 1): K45 T108 I110 W131 C138 I139 V292 S335 K336 I337
• Enzyme Commision number: 4.2.1.112: acetylene hydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C9 S11 C12 I14 N15 C16 C46 K48 S49 W179 V180	C6 S8 C9 I11 N12 C13 C43 K45 S46 W176 V177
BS02	MGD	A	T111 N114 A137 M140 C141 M299 A416 N418 G422 Y423 Y442 D443 Q444 D460 R465 R606 Q612 S613 C614 H676 D699 L719	T108 N111 A134 M137 C138 M296 A413 N415 G419 Y420 Y439 D440 Q441 D457 R462 R603 Q609 S610 C611 H673 D696 L716
BS03	MGD	A	D13 K48 G171 K172 N173 N178 W179 L199 D200 P201 R202 Y218 G219 D221 V295 S296 Q300 F602 L605 R606 E607 D608 N610 F611 Q612 R720	D10 K45 G168 K169 N170 N175 W176 L196 D197 P198 R199 Y215 G216 D218 V292 S293 Q297 F599 L602 R603 E604 D605 N607 F608 Q609 R717

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0016829 lyase activity
• GO:0018818 acetylene hydratase activity
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:2e7z, PDBe:2e7z, PDBj:2e7z
• PDBsum: 2e7z
• PubMed: 17360611
• UniProt: Q71EW5|AHY_SYNAC Acetylene hydratase