Structure of PDB 2e52 Chain A

Receptor sequence

>2e52A (length=299) Species: 71421 (Haemophilus influenzae Rd KW20)

KKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILP
EAIKPSSTQEKLFSKAGDIVLAKAFQLLNLNSKPLEQRGNAGDVIALSKE
FNYGLVADAKSFRLSRTAKNQKDFKVKALSEWREDKDYAVLTAPFFQYPT
TKSQIFKQSLDENVLLFSWEHLAILLQLDLEETNIFSFEQLWNFPKKQSK
KTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEINFIEERSLIEKE
YWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL

3D structure

• PDB: 2e52 Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L49 E60 S64 R88 G89 N90 D93 S111 F112 R113 R116 T117 A118 D123 K125 W132	L49 E60 S64 R88 G89 N90 D93 S111 F112 R113 R116 T117 A118 D123 K125 W132
BS02	dna	A	F20 S56 T58 K61 E86 R88 A118 N120 Q121 K122 P149 T150 T151 K152 S153 Q154 N276	F20 S56 T58 K61 E86 R88 A118 N120 Q121 K122 P149 T150 T151 K152 S153 Q154 N276
BS03	dna	A	N25 I28	N25 I28
BS04	dna	A	Y32 K33	Y32 K33

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity
• GO:0009036 type II site-specific deoxyribonuclease activity

Biological Process

• GO:0009307 DNA restriction-modification system

External links

• PDB: RCSB:2e52, PDBe:2e52, PDBj:2e52
• PDBsum: 2e52
• PubMed: 19966419
• UniProt: P43870|T2D3_HAEIN Type II restriction enzyme HindIII (Gene Name=hindIIIR)