Structure of PDB 2dpi Chain A

Receptor sequence

>2dpiA (length=372) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQVMTPM
VDILMKLFRNMTLLSVCFCNLK

3D structure

• PDB: 2dpi Hoogsteen base pair formation promotes synthesis opposite the 1,N(6)-ethenodeoxyadenosine lesion by human DNA polymerase iota.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E127 K207 I242 G243 K245 T246 E358 S359	E102 K182 I217 G218 K220 T221 E333 S334
BS02	dna	A	Q59 K60 E97 L99 S301 S303 E304 E305 S307 K309	Q34 K35 E72 L74 S276 S278 E279 E280 S282 K284
BS03	MG	A	D34 L35 D126	D9 L10 D101
BS04	MG	A	D34 E127	D9 E102
BS05	DCP	A	L35 D36 C37 F38 Y39 V64 T65 Y68 R71 K77 D126 K214	L10 D11 C12 F13 Y14 V39 T40 Y43 R46 K52 D101 K189

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2dpi, PDBe:2dpi, PDBj:2dpi
• PDBsum: 2dpi
• PubMed: 16819516
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)